Direct detection of verotoxin-producing Escherichia coli in stool samples by PCR
نویسندگان
چکیده
منابع مشابه
Detection of Shiga Toxin-Producing Strains of Escherichia coli (O157:H7) isolated from specimens of urinary and stool by Multiplex-PCR method
Background: Infection of shiga toxin-producing Strains of Escherichia coli has some other dangerous and deadly effects such as hemolytic uremic syndrome (HUS) in addition to diarrhea. Its diagnosis is difficult and there is no information on It's incidence in Iran. The goal of this study is to evaluate and detect shiga toxinproducing strains of E. coli isolated from urinary and stool specimens ...
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Diarrheal diseases continue to be one of the most common causes of morbidity and mortality among young children in developing countries. The objective of this s tudy was to evaluate the multiplex PCR as a rapid diagnostic tool for simultaneous detection of four categories of diarrheagenic E. coli (ETEC, EPEC, EHEC and EAEC) in one PCR reaction using six virulent genes. During the period from Ju...
متن کاملDetection of verotoxin in stool specimens.
A total of 132 fecal specimens containing verotoxin (VT) were subjected to counter-current immunoelectrophoresis (CIE). Of these, 113 (85.6%) were found to be positive by CIE. Another 71 stool specimens containing E. coli serogroup O157 but with flagellar antigens other than H7 were tested for verotoxin by CIE. These stool specimens were negative for VT on Vero cell monolayers. Of these 71 stoo...
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A variety of probiotic bacteria have been tested to control animal and foodborne pathogenic bacteria in livestock. The mechanism of inhibition of pathogenic bacteria for several of those probiotic microorganisms is mediated by the production of bacteriocins. Colicins are probably the group of bacteriocins that have been most thoroughly characterized. Colicins are antimicrobial proteins produced...
متن کاملPrevalence of non-O157:H7 shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska.
We determined the prevalence of Shiga toxin-producing Escherichia coli (STEC) in diarrheal stool samples from Nebraska by three methods: cefixime-tellurite sorbitol MacConkey (CT- SMAC) culture, enterohemorrhagic E. coli (EHEC) enzyme immunoassay, and stx1,2 polymerase chain reaction (PCR). Fourteen (4.2%) of 335 specimens were positive by at least one method (CT-SMAC culture [6 of 14], EHEC en...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1995
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.33.3.519-524.1995